ALP-A is a highly active alkaline phosphatase. It is produced using genetic recombination technology, so it is an enzyme with stable quality.
| Origin | recombinant A. sojae |
|---|---|
| Systematic name | Orthophosphoric-monoester phosphohydrolase (alkaline optimum) |
| EC Number | 3.1.3.1 |
| Reaction formula | An orthophosphoric monoester + H₂O →→→ An alcohol + Orthophosphate |
| Appearance | A clear, slightly yellowish solution |
|---|---|
| Activity | ≧9,000 U/mg protein |
| Protein | 10-20 mg, Bradford assay |
| Storage condition | below -20℃ |
| Molecular weight | ca. 123 kDa (SDS-PAGE) |
|---|---|
| pH Optimum | 9.5-10 (Fig. 1) |
| Thermal stability | below 50℃ (Fig. 2) |
| Optimum temperature | 50℃ (Fig. 3) |
| Thermal stability (liquid form)) | stable at 37℃ for at least one week (Fig. 4) |
| Activators | Mg2+, diethanolamine |
| Inhibitors | Inorganic phosphate, EDTA |
ALP-A is used in immunoassays such as ELISA, immunostaining, and Western blotting by using chromogenic, fluorescent, and chemiluminescent substrates.
