Cholesterol Oxidase (CHO-CE) 胆固醇氧化酶

  • 临床检测用酶
Cholesterol Oxidase胆固醇氧化酶

The enzyme is useful for the determination of cholesterol in clinical analysis.

由来 recombinant E. coli
系统名称

Cholesterol : oxygen oxidoreductase

EC编号 1.1.3.6
反应式

Cholesterol + O2 →→→ Cholest-4-en-3-one + H2O2

规格

Appearance yellow lyophilizate
Activity ≧5 U/mg
Stabilizer trehalose,sodium malate, sodium glutamate, ethylenediaminetetraacetic acid (EDTA)
Storage condition below -20℃ protected from light

特性

Molecular weight ca. 58 kDa (gel filtration)
Structure monomer of 55 kDa (SDS-PAGE)
Michaelis constant 3.5×10-4 M (cholesterol)
pH Optimum Ca. 7.0
pH Stability 5.0–10.0
Optimum temperature 50℃
Thermal stability below 55℃
Stability (liquid form) stable at 37℃ for at least one week
Stability (powder form) stable at 30℃ for at least one month
Inhibitors ionic detergents, Ag+, Hg2+

适用类型

The enzyme is useful for the determination of cholesterol in clinical analysis.

参考文献



TOP