Glutaminase(GLN) 谷氨酰胺酶

  • 临床检测用酶
Glutaminase

The enzyme is useful for the determination of L-glutamine in clinical analysis.

由来 from microorganism
系统名称

L-Glutamine amidohydrolase

反应式

L-Glutamine + H2O →→→ L-Glutamineate + NH3

规格

Appearance light gray lyophilizate
Activity ≧4 U/mg
Contaminants Catalase ≦3.0 U/U%
Stabilizer sucrose
Storage condition below - 20℃

特性

Molecular weight ca. 58 kDa (gel filtration)
Structure heterodimer (45 kDa and 22 kDa) (SDS-PAGE)
Michaelis constant  5.5×10-4 M (L-glutamine)
pH Optimum ca. 7.0
pH Stability 5.5–9.0
Optimum temperature ca. 65℃
Thermal stability below 50℃
Stability (liquid form) stable at 37℃ for at least four days
Stability (powder form) stable at 30℃ for at least two weeks
Specificity L-glutamine (100), D-glutamine (70), L-asparagine (0), D-asparagine (0)

适用类型

The enzyme is useful for the determination of L-glutamine in clinical analysis.

参考文献

Roberts, E., “The Enzymes,” Vol. 4 (2nd ed.), Academic Press, New York and London, 1960, pp. 285–300.

Hartman, S. C., “The Enzymes,” Vol. 4 (3rd ed.), Academic Press, New York and London, 1971, pp. 79–100.

Ayodeji A. et. al. (2019) Recent advances in microbial glutaminase production and applications—a concise review, Crit. Rev. Biotechnol., 39, 944-963



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